LaminarForge

Research context

Useful research tooling starts with reproducible test units.

LaminarForge is building public context around molecular diagnostics, AAV delivery screening, tissue-on-chip workflows, and the first 16-slot cassette concept. This material documents early research infrastructure, not a clinical performance claim or wet-lab protocol.

Why this exists

Start with the workflow before making a performance claim.

TEST
Define the sample and targetA useful assay starts with a specific sample type, molecular target, preparation method, control strategy, and intended result.
DATA
Validate before interpretingTemperature stability, contamination controls, positive and negative controls, timing, and repeatability come before a clinical claim.
AAV delivery

The delivery shell matters before any payload claim matters.

Known fieldOrgan-on-chip is realReusable cassettes, perfused plates, modular fluidics, and AAV-on-chip studies already exist. LaminarForge is not claiming to invent the category.
Open gapThe workflow is still hard to accessMany systems are expensive, proprietary, quote-only, or locked to vendor consumables. The public path from CAD to repeatable validation is often missing.
LaminarForge aimMake the test unit reproducibleThe first target is an open 16-slot cassette workflow: one AAV condition across a cell-type/readout panel, then scale by running matched cassettes.
16-slot cassette

One cassette is one AAV condition across a cell panel.

The cassette is not just a tray. It is the proposed repeatable unit for comparing delivery behavior across matched cell types, cell states, or readouts.

Scale comes from running more matched cassettes: candidate A, candidate B, candidate C, and so on. The goal is not to out-hype existing organ-chip systems. The goal is to make a cheaper, open, inspectable workflow that can be improved in public.

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Validation first

Cells are not software containers.

01Define the measurable questionEach test begins with a specific sample, target, workflow, and acceptance criterion so the result can be interpreted without overclaiming.
02Track delivery selectivityAAV capsids are delivery shells. If future therapies need to reach specific cell types, capsid behavior across relevant cell models needs to be measurable.
03Build a cell-panel cassetteOne cassette represents one AAV condition. The 16 slots hold matched cell types, states, or readouts under the same exposure.
04Validate before biology claimsNo-cell and media-only checks come first: fit, leaks, bubbles, flow balance, environmental logging, imaging access, and run records.
Source trail

Adjacent work to inspect first.

Multiplex viral tropism assay

AAV serotypes across complex human organoid cell populations with single-cell readouts.

Multi-trait AAV capsid engineering

Large capsid libraries and computational screening for delivery traits.

Reusable MPS cassette

Reusable clamped cassette and replaceable inserts for perfused microphysiological systems.

Fluidic circuit board

Modular operation of microfluidic chips for parallel cell culture and liquid dosing.

Emulate AAV organ-chip example

Commercial organ-chip framing for AAV transduction and toxicity work.

AKITA scalable organ-on-chip platform

Plate-format organ-on-chip platform with standardized layout and TEER readouts.